Separation of The Chemical Composition of Proteins in The Human Body

The article majorly discusses the technique that is undertaken when it comes to separation of the chemical composition of proteins in the human body of a healthy man or woman from a sick individual so that appropriate information can be got and used to assign an person the appropriate medication options. The take a look at that are currently existing are concerned with the diagnosis of neuroendocrine tumors (NETs), which are regarded as organic markers. However, the process is regarded as a complicated one as it makes it very hard for the detection of developing most cancers cells within an individual. The methods that have been used in the system of separation and extraction of these proteins are very few but are vital in the process. The method is known as the ID PAGE (one-dimensional polyacrylamide gel electrophoresis). The process begins with the extraction of the infected section (tumors), followed by the section be cultured in both the petri dish and RPMI-1640. The result of these is then filtered and treated. The resultant products are then centrifuged and tested as per the recommendation of the test. The one-dimensional polyacrylamide gel electrophoresis technique is one that uses the NET samples that have been collected from a variety of tumor locations within the body and are tested against similarly collected control samples. The procedure has about 5 steps that are used in getting the required findings. The first process involves the isolation of the neuroendocrine tumors. The collection of these samples are collected during a surgery in a sterilized environment to avoid contamination of the samples. Once the samples have been collected, they are cultured in RPMI-1640. After the second process has been completed, the samples are then taken to the third process which involves the samples being cultured in petri dishes. The samples that are used in this process are however reduced in their composition of fats and tissue so that they can be easily crushed, filtered through the various cell strainers before the filtrates are subjected osmotic shock and being centrifuged. The main reason for this specific procedure is to allow and ensure that the erythrocytes in the samples have undergone complete hemolysis. The pellets are then collected after this process and are subjected to the fourth procedure which is basically a form of counting and redistributing the processed samples in terms of their individuality as cells. The last process involve collection of supernatants from the cultured specimens after a period of about three days (72 hours). The supernatants are then centrifuged and distributed in a similar manner just as that in procedure 4. During the third procedure, it should be noted that the main reagent that was used is acetone-methanol. The main reason being that the reagent allows for the desalinization and concentration of the samples which will allow for the formation of the pellets. During testing, the SDS - Page technique involved the thawing and mixing of samples with a buffer comprising glycerol, Tris-HCl, β-mercaptoethanol, SDS and bromophenol blue. The glycerol’s role in the mixture is to increase the density of the mixture while the role of the bromophenol blue was to act as a staining agent in the specimen in order to allow the researchers to observe the process of protein separation. The buffer would then be incubated and centrifuged.