Cellular morphogenesis in Dictyota dichotoma

It was carried out to see if cell elongation contributed to cell growth. Positive size change is one example.
Membrane depolarization, microscopy, staining, and a combination of pharmacological techniques are among the techniques used. Thalli were grown in the aerated 1 L culture flasks overnight in the dark using modified seawater rich in Provasoli at a temperature of 19 degrees Celsius. The male and female gametophytes later discharged the gametes.
The method utilized is extremely quick to display the results. From the experiment done, the outcome is seen after 90 seconds.







Observations made

The lengthening of the zygote is not part of growth. F- actin and myosin are the ones responsible, secretion is very vital but must be accompanied brefeldin. The shape and size change after 90 seconds.



Interpretation of the observation by author

The author observes that cell morphogenesis is brought about by size increase. He also discovers that microtubular cytoskeleton is not involved in the generation of the force responsible for the lengthening.

Critical interpretation of data

The data support the conclusion made because in the experiment it is observed that using myosin and F- actin makes the fertilised egg of Dictyota to have very fast growth. The process is then regulated using cytoplasmic calcium. Hence the conclusion of the author is, the elongation is not part of growth.



Alternative interpretation of data

Alternative observations cannot be accounted for because observations made are the ones used in the interpterion of the data given.



Alternative approaches that could be used

The experiment can be successful by inducing membrane depolarisation that is, seawater rich in potassium and calcium influx.

Reference

Bogaert, Kenny A., Tom Beeckman, and Olivier De Clerck. "Egg activation-triggered shape change in the Dictyota dichotoma (Phaeophyceae) zygote is actin–myosin and secretion dependent." Annals of Botany (2017).